Highly sensitive antibody-free quantitation of rhTRAIL in serum using two-dimensional SPE and μLC-MS/MS
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منابع مشابه
Antibody-free LC-MS/MS quantification of rhTRAIL in human and mouse serum.
The major challenge in targeted protein quantification by LC-MS/MS in serum lies in the complexity of the biological matrix with regard to the wide diversity of proteins and their extremely large dynamic concentration range. In this study, an LC-MS/MS method was developed for the simultaneous quantification of the 60-kDa biopharmaceutical proteins recombinant human tumor necrosis factor-related...
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